Composite

Part:BBa_K3859014:Design

Designed by: TianYi Huang   Group: iGEM21_GreatBay_SZ   (2021-10-01)


Ura3 upstream homologous region-Promoter-sfGFP-terminator-Ura3 downstream homologous region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3
    Illegal NotI site found at 3523
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1842
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1731
    Illegal BsaI.rc site found at 713
    Illegal SapI.rc site found at 897
    Illegal SapI.rc site found at 2628


Design Notes

atcg


Source

atcg

References